DMPK Studies
DMPK Studies

DMPK Studies


Preclinical (DMPK) studies

In Vitro ADME

  • Aqueous solubility and stability of the compounds at different pH, Stability of the compounds at ambient temperature, 2-8/-20°C
  • Short term and long term stability of the compound in biological matrices(plasma/serum) Metabolic stability studies using cellular (hepatocytes from rat, mouse, human etc) and sub cellular fractions, metabolic stability studies recombinant CYP isoforms such as CYP3A4, CYP2C9, CYP2C19, CYP2D6 and CYP1A2
  • Cell permeability assays – PAMPA, MDCK, and CaCo-2
  • Plasma/serum protein binding by ultrafiltration / equilibrium dialysis technique

In-Vivo DMPK services

  • Pre-formulation development
  • Absolute Bioavailability studies
  • Single, Multiple and cassette dosing
  • Ascending dose PK in rodents
  • PK studies in cannulated and non-cannulated rodents

In vivo models

  • Inflammatory pain: CFA, Carrageenan, Capsaicin, Formalin models, collagen-induced arthritis
  • IBD: DSS induced colitis in mice, TNBS induced colitis in rats
  • Respiratory: Rodent models of LPS-induced neutrophilia and ovalbumin induced eosinophilia
  • Cytokine Release Models: Mouse models of LPS induced TNF α and IL-1 release, rat model of ATP activated IL-1β release
  • Acute and Chronic OGTT, IPGTT models (glucose & insulin)
  • DIO mouse model (pair fed studies)
  • Acute model for GLP-1 induced Incretin secretion & endocrine secretion (PYY / CCK)
  • Acute model for Malonyl CoA lowering