DMPK Studies
Preclinical (DMPK) studies
In Vitro ADME
- Aqueous solubility and stability of the compounds at different pH, Stability of the compounds at ambient temperature, 2-8/-20°C
- Short term and long term stability of the compound in biological matrices(plasma/serum) Metabolic stability studies using cellular (hepatocytes from rat, mouse, human etc) and sub cellular fractions, metabolic stability studies recombinant CYP isoforms such as CYP3A4, CYP2C9, CYP2C19, CYP2D6 and CYP1A2
- Cell permeability assays – PAMPA, MDCK, and CaCo-2
- Plasma/serum protein binding by ultrafiltration / equilibrium dialysis technique
In-Vivo DMPK services
- Pre-formulation development
- Absolute Bioavailability studies
- Single, Multiple and cassette dosing
- Ascending dose PK in rodents
- PK studies in cannulated and non-cannulated rodents
In vivo models
- Inflammatory pain: CFA, Carrageenan, Capsaicin, Formalin models, collagen-induced arthritis
- IBD: DSS induced colitis in mice, TNBS induced colitis in rats
- Respiratory: Rodent models of LPS-induced neutrophilia and ovalbumin induced eosinophilia
- Cytokine Release Models: Mouse models of LPS induced TNF α and IL-1 release, rat model of ATP activated IL-1β release
- Acute and Chronic OGTT, IPGTT models (glucose & insulin)
- DIO mouse model (pair fed studies)
- Acute model for GLP-1 induced Incretin secretion & endocrine secretion (PYY / CCK)
- Acute model for Malonyl CoA lowering