Pain and Inflammation

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At Skanda Life Sciences, our pain and inflammation research services are dedicated to accelerating drug discovery through a robust portfolio of in vitro assays and pre-clinical models

Pain and Inflammation

At Skanda Life Sciences, our pain and inflammation research services are dedicated to accelerating drug discovery through a robust portfolio of in vitro assays and pre-clinical models. Our comprehensive approach spans from inflammatory cell cultures to in vivo models, enabling thorough evaluation of potential anti-inflammatory and analgesic agents. With a commitment to innovation and collaborative partnerships, our goal is to pioneer novel therapeutics targeting pain and inflammation, driving advancements in drug discovery and pre-clinical evaluation.

For Pain and Inflammation, here are some common assays and their associated cell lines:

Inflammatory Cytokine/Chemokine Release Assays

  • THP-1: Human monocytic leukaemia cells (often differentiated into macrophage-like cells)
  • RAW 264.7: Murine macrophage cell line
  • Peripheral blood mononuclear cells (PBMCs): Isolated from human blood and contain various immune cell types
  • Assay: ELISA (Enzyme-Linked Immunosorbent Assay) for quantifying cytokine secretion (e.g., TNF-α, IL-6, IL-1β, Etc.)
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COX Inhibition Assay:

  • Cell Line: RAW 264.7, THP-1
  • Assay: Measurement of COX-2 enzyme inhibition using specific substrates (e.g., Prostaglandin E2 production assay)

NF-κB Activation Assay:

  • Cell Line: HEK293 (Human embryonic kidney cells)
  • Assay: Reporter gene assays or luciferase assays to measure NF-κB activation upon stimulation with inflammatory mediators

Cell Migration Assay:

  • Cell Line: Human dermal fibroblasts, Human umbilical vein endothelial cells (HUVECs)
  • Assay: Wound healing assay or transwell migration assay to assess the migratory capacity of cells in response to inflammatory stimuli

Reactive Oxygen Species (ROS) Assay:

  • Cell Line: RAW 264.7, THP-1
  • Assay: Detection of intracellular ROS levels using fluorescent probes such as DCFDA (2′,7′-Dichlorofluorescin diacetate)

These assays can help evaluate the anti-inflammatory properties of compounds and assess their potential as therapeutics for Pain and Inflammation-related conditions.