In vitro and In vivo Assessment of Immunomodulatory studies against Zika Virus, Influenza A, & HCoV-229E
Virology
- In vitro and In vivo Assessment of Immunomodulatory studies against Zika Virus, Influenza A, & HCoV-229E
- Cytokine levels –IgG, IgM, IL-6, IFN-γ, and TNF-α, IL-2, IL-12, IL-10
- Immuno-phenotyping of cells by flowcytometry –T cells, B cells, NK cells
- Haematological, biochemical and Histopathology of organs
- In-vitro neutralization assay
- In-vitro immunogenicity using PBMCs
- Cytotoxic evaluation of novel anti-viral peptide in VERO cells
- Plaque Reduction Assay
- In vivo efficacy of novel anti-viral
- Effect of novel anti-viral peptide in preventing the viral protein synthesis
- Cytopathic effect for antiviral compounds/ Formulation
- Relative expression/ identification by using RTPCR method
- Screening of antiviral compounds/Vaccines both in cell lines & animal models
