At Skanda Life Sciences, we are keen on progressing osteoporosis research through various in vitro methodologies.
Osteoporosis
At Skanda Life Sciences, we are keen on progressing osteoporosis research through various in vitro methodologies. Our comprehensive approach combines innovative cell culture techniques with appropriate assays to elucidate the underlying mechanisms of osteoporosis and accelerate the development of novel therapeutic interventions.Our collection includes a diverse range of osteoblast and osteoclast cell lines, each carefully selected to mimic the cellular processes involved in bone metabolism. These cell lines, including SaOS-2, RAW 264.7, and UMR-106, that serve as invaluable tools for studying osteoblast differentiation, osteoclast formation, etc.,
In Vitro Assays
We employ a variety of in vitro assays to investigate key aspects of osteoporosis pathology and evaluate potential therapeutic candidates. These assays include:
Alkaline Phosphatase Activity Assay
Measures osteoblast activity by quantifying alkaline phosphatase production, a critical marker of bone formation.
Tartrate-Resistant Acid Phosphatase (TRAP) Assay
Assesses osteoclast activity by quantifying TRAP expression, indicative of bone resorption.
Mineralization Assay
Evaluates osteoblast function by assessing calcium deposition and mineralized bone matrix formation.
Osteoclastogenesis Assay
Studies osteoclast differentiation induced by key regulators such as RANKL and M-CSF, providing insights into bone resorption processes.
Gene and Protein Expression Assays
Evaluate the expression of genes and proteins involved in bone metabolism, such as osteocalcin, collagen type I, Runx2, and RANKL.
Signaling Pathway Assays
Investigate the activation of signaling pathways involved in bone formation and resorption, such as Wnt/β-catenin, BMP, and MAPK pathways.
