SKANDA provides a wide range of services in the area of recombinant DNA technology, strain engineering and gene expression analysis.

Gene Expression Analysis

Expression of a targeted gene at the transcriptional and translational levels in a host system is important in studying the functional gene. Scientists at routinely carry out the following gene expression analysis:

Real Time PCR
Western blotting
ELISA
Flow Cytometry
Mass spectrometry
RNA sequencing
Molecular Biology

Automated DNA sequencer- ABI 3730 XL, ABI 3130XL
Thermal Cyclers
Real-Time PCR instruments (Biorad & ABI)
Pulsed field electrophoresis
Gel Documentation Systems
Nano Drop Spectrophotometer
Microbiology

Bench Top Fermenters
Biosafety Cabinets- BSL I & II
Incubators and Incubator Shakers
Brief Services

Gene expression studies by PCR, RT-PCR and qPCR methods.
DNA cloning, DNA sequencing, gene expression and western blotting services.
Cancer gene expression study Caspase3, Caspase8, Caspase9 BCL2, Bax, Brac 1, Brac 2, HSP90 and HSP27.
RAPD, Next generation sequencing, transfection
Native PAGE, SDS, PAGE, 2D electrophoresis, Protein expression
Transfection of clones in mammalian cell lines CHO-K1, HEK 293, 3T3, MCF7 etc.
Clonal selection, amplification, analysis of expression by various Biochemicals and functional methods.
16s and 18s rRNA sequencing and phylogenic analysis.
Antibody Production & Purification

Skanda is the preferred antibody production who leverages our scientific expertise and flexibility for high quality. Skanda tailored experienced scientists offer small and large scale monoclonal and polyclonal antibody production and purification. We also offer hybridoma generation and screening services.

Polyclonal

Polyclonal antibodies production – New Zealand White Rabbit standard or custom requirement
Variety of antigens (bacterial, fusion proteins & peptides)
Hybridoma

Immunization of mice/rat
Conjugation for immunization
Animal care & handling
Hybridoma generation and screening by ELISA and Biacore
Screening for biological (neutralization, internalization etc.)
Titer determination, Sub cloning and isotyping of selected clones
Purification and analysis of monoclonal antibodies
Cryopreservation of cell lines
Recombinant MAbs

Transient transfections (in HEK293) and/ or stable cell line generation (in CHO-based expression systems)
Cloning of antibody genes from hybridoma and generation of high-expressing plasmid constructs
Production from small scale to large scale cultures
Purification of antibodies, Cell banking and quality control