Skanda is dedicated to advancing cardiovascular disease research through innovative pre-clinical services. Our expertise covers a range of in vitro assays and models to study CVD pathophysiology and evaluate potential therapeutics.
Key assays and models include:
- Endothelial Cell Cultures: Endothelial cell cultures, such as HUVECs cells, are employed to investigate endothelial dysfunction, vascular inflammation, atherosclerosis. and vascular permeability. These cultures facilitate the study of endothelial barrier function, angiogenesis, and responses to vasoactive agents and inflammatory mediators.
- Cardiomyocyte Cultures: Enable the study of cardiomyocyte function, contractility, and responses to stressors. Primary or induced pluripotent stem cell-derived cardiomyocytes, such as H9c2 cells or HL-1 cells, are utilized to study various aspects of cardiac function
- Platelet Aggregation Assays: Assess platelet function and thrombogenic potential in the context of CVD and antiplatelet therapies.
- Atherosclerosis Models: Utilize endothelial cell cultures and macrophage foam cell formation assays to study atherosclerosis development and progression.
- Skanda leverages sophisticated technologies such as Flow Cytometry, ELISA, and qPCR to analyze cell behavior, cytokine secretion, and gene expression profiles in cardiovascular research. Through these approaches, we aim to elucidate the mechanisms underlying CVD and accelerate the development of novel therapeutics
Experimental Technologies
Experimental techniques such as Flow Cytometry, Fluorescence Microscopy, qPCR, and Western Blot are employed to assess cellular function and signalling pathways.
This tailored approach enables us to advance our understanding of cardiovascular disease mechanisms and develop and identify promising drug candidates, elucidate disease pathways, and ultimately develop effective treatments for cardiovascular diseases.
